Differential expression of transcription factors in CD8⁺ T cells isolated at week 5 and week 20 post-vaccination with SIVΔnef and at week 20 post-infection with wild-type SIV.

<p>Symbols indicate log₂ expression relative to endogenous controls in cells from individual animals. Red symbols indicate Gag CM9-specific cells, blue symbols indicate Tat SL8-specific cells. Sample means are indicated by horizontal bars. Statistically significant (p≤0.05) differences in transcription factor expression between cells from week 5 and week 20 post-SIVΔnef vaccination, or between cells from week 20 post-SIVΔnef vaccination and week 20 post-wild-type SIV infection are indicated by horizontal bars with asterisks. Statistically significant differences between Gag CM9 and Tat SL8-specific cells are indicated by vertical bars with asterisks.</p

An application of HOMER and ACMANT for homogenising monthly precipitation records in Ireland.

Climate change studies based only on raw long-term data are potentially flawed due to the many breaks introduced from non-climatic sources, consequently quality controlled and homogenised climate data is desirable for basing climate related decision making on. Seasonal cycles of precipitation in Ireland and the UK are projected to become more marked as the climate changes, and regional extremes in summer dry spells and winter precipitation have been recorded in recent years. Therefore to analyse and monitor the evolution of precipitation patterns across Ireland, quality controlled and homogenous climate series are needed

Principal component analysis heat maps of transcription factor expression profiles from SIV-specific MHC tetramer-sorted CD8⁺ T cells from animals vaccinated with SIVΔnef, and sorted naïve and memory CD8⁺ T cells.

<p>Heat map log₂ relative expression values were range normalized for each transcription factor.</p

Differential expression of transcription factors in CD8⁺ naïve and memory T cell subsets.

<p>log₂ mean expression values were normalized to naïve cell samples.</p

Transcription factors analyzed in bulk and SIV-specific CD8⁺ T cells.

<p>Transcription factors analyzed in bulk and SIV-specific CD8⁺ T cells.</p

Principal component analysis of transcription factor expression profiles from SIV-specific MHC tetramer-sorted CD8⁺ T cells and sorted CD8⁺ T cell subsets.

<p>(<b>A</b>) Plot of principal components 1 vs. 2, and 2 vs. 3 for each of the expression profiles assessed in sorted naïve and memory CD8⁺ T cell subsets isolated from healthy control animals (n = 5), and SIV-specific MHC tetramer-sorted CD8⁺ T cells isolated from animals (n = 4) at week 5 or week 20 following SIVΔnef vaccination. Principal components 1, 2 and 3 explain 92% of cumulative total variance. (<b>B</b>) PCA loading factors for each transcription factor.</p

Principal component analysis of transcription factor expression profiles from SIV-specific MHC tetramer-sorted CD8⁺ T cells from animals vaccinated with SIVΔnef, animals infected with wild-type SIV, and sorted CD8⁺ T cell subsets.

<p>Plot of principal components 1 and 2 for each of the expression profiles assessed from sorted naïve and memory CD8⁺ T cell subsets, SIV-specific MHC tetramer-sorted CD8⁺ T cells isolated from SIVΔnef-vaccinated animals, and MHC tetramer-sorted CD8⁺ T cells isolated from animals (n = 5) at 20 weeks following wild-type SIV infection. Principal components 1 and 2 explain 77 percent of cumulative total variance.</p